| Biography | |
|---|---|
 Prof. Nanguang Chen National University of Singapore, Singapore |
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| Title: Combining multiphoton fluorescence microscopy with focal modulation for improved penetration depth | |
| Abstract: Nonlinear fluorescence microscopy methods, including two-photon and three-photon microscopic methods, have become increasingly popular in biomedical researches. Due to highly selective excitation of fluorophores, nonlinear light microscopes enjoy a much-improved imaging depth than single photon fluorescence microscopes. With the help of low-repetition femtosecond lasers, some groups have achieved greater than 1 mm penetration depths in mouse brain. We have been investigating the potential of combining nonlinear light excitation with focal modulation in attempt to further reduce the strong background and bring the penetration depth to 2 mm and above. We have focused on design issues of spatiotemporal phase modulator, a key component for additional background rejection. Various designs have been evaluated and optimized to achieve the best combination of modulation depth, background modulation, as well as spatial resolution. | |
| Biography: Dr. Nanguang Chen is currently an Associate Professor of Biomedical Engineering at the National University of Singapore (NUS). He received his PhD in Biomedical Engineering in 2000 from Tsinghua University. He also received his MSc in Physics and BSc in Electrical Engineering in 1994 (Peking University), and 1988 (Hunan University), respectively. He joined the Optical and Ultrasound Imaging Lab at the University of Connecticut in 2000 as a postdoctoral fellow and then became an Assistant Research Professor in 2002. Since 2004, he has been a faculty member with NUS. His research interests include diffuse optical tomography, optical coherence tomography, and novel microscopic imaging methods. | |
